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정보제공 : ZEUS 장비활용종합포털(www.zeus.go.kr)

겔침투크로마토그래프

Gel Permeation Chromatograph

GBTP No. GBREMS-27-0134
NFEC등록번호 NFEC-2007-10-005821
I-tube등록번호 -
보유기관 경북대학교
연락처 054-530-5202

장비정보

모델명 2695 제작사 Waters
취득일자 2003-05-22 취득금액 77,354,880원
표준분류 화합물전처리/분석장비 > 분리분석장비 > 액체크로마토그래피 활용용도 시험
장비위치
경상북도 상주시 경상대로 2559 (가장동) 상주캠퍼스 상주캠퍼스 8호관 F1 8404-1
장비설명 Alliance System
구성 및 기능 High-performance liquid chromatography (or high-pressure liquid chromatography HPLC) is a chromatographic technique that can separate a mixture of compounds and is used in biochemistry and analytical chemistry to identify quantify and purify the individual components of the mixture.

HPLC typically utilizes different types of stationary phases a pump that moves the mobile phase(s) and analyte through the column and a detector that provides a characteristic retention time for the analyte. The detector may also provide other characteristic information (i.e. UV/Vis spectroscopic data for analyte if so equipped). Analyte retention time varies depending on the strength of its interactions with the stationary phase the ratio/composition of solvent(s) used and the flow rate of the mobile phase. It is a form of liquid chromatography but smaller column size smaller beads inside column and higher pressures.

With HPLC a pump (rather than gravity) provides the higher pressure required to propel the mobile phase and analyte through the densely packed column. The increased density arises from smaller particle sizes. This allows for a better separation on columns of shorter length when compared to ordinary column chromatography.
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